| Place | Lane | Name | Affiliation | Time |
| 1 | 3 | Ravin Alexander | Eagles | 38.52 |
| 2 | 1 | Semira Killebrew | Indy Racing | 38.71 |
| 3 | 7 | Ceana Carey | Eagles | 43.00 |
| 4 | 4 | Ta'lor Washington | Lima Striders | 45.47 |
| 5 | 2 | Slyvia Upthegrove | Lima Striders | 45.50 |
| 6 | 6 | Aerica Bennett | Lima Striders | 52.84 |
| SCR | 5 | Londyn Niss | Blessed To R | |
| SCR | 8 | Ja'neisha Brown | Eagles |